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Visual literacy is recognized as a threshold concept in biochemistry and molecular biology. However, a consensus on the optimal methods for teaching and evaluating remains elusive. For a decade, BioMolViz has strived to enhance biomolecular visualization assessment. Through workshops and online working groups, we guide instructors on how to probe biomolecular visual literacy using accessible images and questions, which are ultimately shared broadly through our online repository (the BioMolViz Library). Here, we present the final step of our assessment validation process which occurred during the 2022-2023 academic year. We engaged life science students from seven U.S.-based institutions in a pilot field test. Students responded to the multiple choice, multiple select and free response items, rated them on their perceived difficulty, and provided optional open-ended feedback. As we examined the data, we became curious about whether instructors viewed the difficulty level of the items similarly to students. We followed up with an instructor survey where respondents rated and commented on the difficulty of 14 assessment items that were administered to students in the pilot field test. Subsequently, we conducted a mixed methods study to analyze our quantitative and qualitative data. Our analysis revealed a statistically significant disparity between instructors' and students' perceptions of assessment difficulty. Notably, regression models suggest that students' performance predicts their perceived difficulty, with high-performing students finding the assessment generally easier than their lower-performing peers. This points to the crucial role of performance in shaping students' perceptions, while also indicating that instructors, on the whole, tended to view the assessment as less challenging than students. To gain deeper insights into these findings, we performed thematic coding of both student and instructor responses. Our analysis unveiled three pivotal themes in visual literacy assessment: (a) expectations about images guide student performance, (b) disparities exist in visual literacy problem solving, and (c) content knowledge can be both a help and hindrance in visualization. Importantly, these results have changed the way members of our team now approach teaching and evaluating biomolecular visualization skills in our own classrooms. We will share our revised approaches alongside results from our study and provide practical recommendations to aid educators in effectively teaching and evaluating visual literacy in their classrooms. This material is supported by the National Science Foundation (NSF) under grants RCN-UBE #1920270 and NSF-IUSE #1712268 

While visual literacy has been identified as a foundational skill in life science education, there are many challenges in teaching and assessing biomolecular visualization skills. Among these are the lack of consensus about what constitutes competence and limited understanding of student and instructor perceptions of visual literacy tasks. In this study, we administered a set of biomolecular visualization assessments, developed as part of the BioMolViz project, to both students and instructors at multiple institutions and compared their perceptions of task difficulty. We then analyzed our findings using a mixed-methods approach. Quantitative analysis was used to answer the following research questions: (1) Which assessment items exhibit statistically significant disparities or agreements in perceptions of difficulty between instructors and students? (2) Do these perceptions persist when controlling for race/ethnicity and gender? and (3) How does student perception of difficulty relate to performance? Qualitative analysis of open-ended comments was used to identify predominant themes related to visual problem solving. The results show that perceptions of difficulty significantly differ between students and instructors and that students’ performance is a significant predictor of their perception of difficulty. Overall, this study underscores the need to incorporate deliberate instruction in visualization into undergraduate life science curricula to improve student ability in this area. Accordingly, we offer recommendations to promote visual literacy skills in the classroom. 

Biochemistry relies heavily on the use of images and diagrams to make the abstract tangible. Yet, as biochemistry instructors, how do we know whether our students see the same things we instructors see? BioMolViz (biomolviz.org) is a community of practice dedicated to the instruction and assessment of biomolecular visual literacy. To this end, BioMolViz created the Biomolecular Visualization Framework, an assessment tool that identifies more than 200 learning objectives clustered under twelve overarching themes, such as StructureFunction and Alternate Renderings. The team is currently assembling a searchable repository to host assessment instruments for each of these learning objectives. To aid in its construction, BioMolViz is recruiting participants to help write, revise, and pilot these instruments in the classroom. Here, we introduce the Framework, share various BioMolViz projects, and invite likeminded individuals to work with us to build students’ biomolecular visual literacy. 

Abstract While molecular visualization has been recognized as a threshold concept in biology education, the explicit assessment of students' visual literacy skills is rare. To facilitate the evaluation of this fundamental ability, a series of NSF‐IUSE‐sponsored workshops brought together a community of faculty engaged in creating instruments to assess students' biomolecular visualization skills. These efforts expanded our earlier work in which we created a rubric describing overarching themes, learning goals, and learning objectives that address student progress toward biomolecular visual literacy. Here, the BioMolViz Steering Committee (BioMolViz.org) documents the results of those workshops and uses social network analysis to examine the growth of a community of practice. We also share many of the lessons we learned as our workshops evolved, as they may be instructive to other members of the scientific community as they organize workshops of their own.